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embryonic mouse fibroblast cell line nih3t3  (ATCC)


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    ATCC embryonic mouse fibroblast cell line nih3t3
    Embryonic Mouse Fibroblast Cell Line Nih3t3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 13280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nih+3t3+mouse+fibroblast+cells/NIH%2F3T3/10__1007_slash_s12195___026___00945___7-81-0-9
    Average 99 stars, based on 13280 article reviews
    embryonic mouse fibroblast cell line nih3t3 - by Bioz Stars, 2026-10
    99/100 stars

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    Cell Culture:

    Article Title: Organ-Specific Metabolite Profiling of Mahonia aquifolium (Pursh) Nutt. Extracts by GC-FID/MS and UHPLC-HRMS/MS with Bioactivity Assessment
    Article Snippet: .. NIH/3T3 mouse fibroblast cells (ATCC, CRL-1658) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin until they reached 70–80% confluence. .. Similarly, human monocyte THP-1 cells (ATCC, TIB-202) were maintained in Roswell Park Memorial Institute (RPMI) medium supplemented with 10% FBS, 1% penicillin–streptomycin, and 0.05 mM 2-mercaptoethanol (Gibco, 2198–023) under 5% CO 2 at 37 °C until 70–80% confluence was achieved.

    Article Title: Plasma‐Processed Bioprinted Chitosan Hydrogels: Enhancing Physicochemical and Antibacterial Properties for Wound Healing
    Article Snippet: .. NIH/3T3 mouse fibroblast cells (ATCC) were cultured in a 50/50 mixture of DMEM high glucose and Ham's F12 with L‐glutamine, supplemented with 10% FBS and 1% PS. ..

    Modification:

    Article Title: Organ-Specific Metabolite Profiling of Mahonia aquifolium (Pursh) Nutt. Extracts by GC-FID/MS and UHPLC-HRMS/MS with Bioactivity Assessment
    Article Snippet: .. NIH/3T3 mouse fibroblast cells (ATCC, CRL-1658) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin until they reached 70–80% confluence. .. Similarly, human monocyte THP-1 cells (ATCC, TIB-202) were maintained in Roswell Park Memorial Institute (RPMI) medium supplemented with 10% FBS, 1% penicillin–streptomycin, and 0.05 mM 2-mercaptoethanol (Gibco, 2198–023) under 5% CO 2 at 37 °C until 70–80% confluence was achieved.

    Polymer:

    Article Title: Agro-Industrial Waste Valorization for Sustainable PHBV Production from Sugarcane Bagasse Using Bacillus sp. HLI02.
    Article Snippet: .. The cytotoxicity of the polymer was checked on NIH/3T3 mouse fibroblast cells (ATCC CRL 1658) through viability (MTT) assay. .. Cells were grown in Dulbecco’s Modified Eagle Medium containing 1.5 g/L glucose (DMEM-Low Glucose, HiMedia, India), added with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA) and 1% antibiotic (100 U/mL of penicillin and 100 μg/mL streptomycin; from Hi-Media, India) in the incubation was maintained at 37 ◦C with 5% CO2 and 95% RHS.

    Article Title: Agro-Industrial Waste Valorization for Sustainable PHBV Production from Sugarcane Bagasse Using Bacillus sp. HLI02
    Article Snippet: .. The cytotoxicity of the polymer was checked on NIH/3T3 mouse fibroblast cells (ATCC CRL 1658) through viability (MTT) assay. .. Cells were grown in Dulbecco’s Modified Eagle Medium containing 1.5 g/L glucose (DMEM-Low Glucose, HiMedia, India), added with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA) and 1% antibiotic (100 U/mL of penicillin and 100 μg/mL streptomycin; from Hi-Media, India) in the incubation was maintained at 37 °C with 5% CO 2 and 95% RHS.

    MTT Assay:

    Article Title: Agro-Industrial Waste Valorization for Sustainable PHBV Production from Sugarcane Bagasse Using Bacillus sp. HLI02.
    Article Snippet: .. The cytotoxicity of the polymer was checked on NIH/3T3 mouse fibroblast cells (ATCC CRL 1658) through viability (MTT) assay. .. Cells were grown in Dulbecco’s Modified Eagle Medium containing 1.5 g/L glucose (DMEM-Low Glucose, HiMedia, India), added with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA) and 1% antibiotic (100 U/mL of penicillin and 100 μg/mL streptomycin; from Hi-Media, India) in the incubation was maintained at 37 ◦C with 5% CO2 and 95% RHS.

    Article Title: Agro-Industrial Waste Valorization for Sustainable PHBV Production from Sugarcane Bagasse Using Bacillus sp. HLI02
    Article Snippet: .. The cytotoxicity of the polymer was checked on NIH/3T3 mouse fibroblast cells (ATCC CRL 1658) through viability (MTT) assay. .. Cells were grown in Dulbecco’s Modified Eagle Medium containing 1.5 g/L glucose (DMEM-Low Glucose, HiMedia, India), added with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA) and 1% antibiotic (100 U/mL of penicillin and 100 μg/mL streptomycin; from Hi-Media, India) in the incubation was maintained at 37 °C with 5% CO 2 and 95% RHS.



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